The Department of Pediatrics is one of the largest departments within the School of Medicine with approximately 171 Faculty, 60 post-doctoral fellows (both MDs and PhDs) along with over 300 support staff (not including hospital staff). In addition, the Department has 57 clinical residents and fellows distributed across the Divisions. The missions of research, education, and patient care are intertwined and are integral to the goals of the department.

Under supervision, the Research Assistant assists with technical procedures in the laboratory regarding cancer immunology. Assists with mouse colony management and genotyping using established PCR techniques. Works with small animals and assist with procedures such as injections and monitoring pregnancies. Assists with various cell culture techniques such as performing flow cytometry analysis of cell surface protein expression, cell propagation, contaminant testing, and cryopreservation. Assist with immunofluorescence staining of frozen and fixed tissues or cells. Assists with protein biochemistry techniques including protein quantification, SDS PAGE, immunoprecipitation, and Western blotting. Maintain and prepare accurate records of all experiments. Assists in the preparation of data for publication. Performs other duties as assigned.

• Theoretical knowledge of molecular biology, immunology, cell biology, and/or biochemistry.

• Ability to perform molecular biology techniques such as PCR, RT-PCR, cDNA synthesis, gene expression analysis, recombinant protein expression, RNA/DNA isolation, protein purification.

• Ability to perform cell biology techniques including: mammalian cell culture, DNA transfection, cell fractionation and differential centrifugation, fluorescence- and luminescence-based bioassays.

• Experience in immunology, including ability to perform immunofluorescence assays, ELISA, immunoprecipitation, co-immunoprecipitation.

• Ability to perform light, fluorescence and confocal microscopy techniques to visualize protein expression in tissue sections.

• Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment, as well as to effectively and accurately document research procedures. Ability to read, comprehend, and discuss research materials.

• Ability and willingness to handle and work with small animals. Experience to assist with mouse genotyping using DNA isolation, established PCR-based methods and argarose gel electrophoresis. Ability to assist in performing mouse experiments and injections.

• Basic understanding of computer applications such as Word, Excel and Powerpoint. Internet search and software analysis capabilities are preferred. Knowledge and experience with PRISM preferred.

• Ability to learn to use histological and immunofluorescence techniques to evaluate protein localization in fixed cells or tissues.

• Ability to perform sterile eukaryotic cell culture techniques.

• Familiarity with basic laboratory equipment such as centrifuges, spectrophotometers, balances, pH meters, autoclaves, and electrophoresis equipment.

• Ability to learn to perform DNA and protein characterization techniques using electrophoretic analysis.

• Familiar with proper handling of chemical and biohazard materials. Familiarity with sterile techniques.

• Ability to learn to handle mouse tissues from harvest to immunostaining.

• Enthusiasm for learning and a genuine interest in scientific research. Willingness and ability to learn new skills, research methods, and scientific principles.

• Detail-oriented. Ability to work with little supervision. Ability to follow verbal and/or written instructions. Ability to follow-up on previously begun procedures. Ability to accurately record data and enter into appropriate medium.

• Employment is subject to a criminal background check.

• Must be willing and able to work with hazardous materials (biological and chemical).

• Must be willing and able to work with small animals.